Toc75 Antikörper

Produktnummer ABIN488514

Dieses Anti-Toc75-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Toc75 in FACS, IL und WB. Geeignet für Pisum sativum, Synechococcus sp., Thermosynechococcus und Synechocystis.

Kurzübersicht für Toc75 Antikörper (ABIN488514)

Target: Toc75 (Chloroplast Outer Envelope Membrane Translocon Complex Oep75 Protein (Toc75))

Reaktivität: Pisum sativum, Synechococcus sp., Thermosynechococcus, Synechocystis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Toc75 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunolocalization (IL), Western Blotting (WB)

Produktdetails anti-Toc75 Antikörper

Kreuzreaktivität (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Homologie: Ricinus communis, Vitis vinifera, Populus trichocarpa

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: 88 / 75 kDa (ocassionally a processing intermediate at 78 kDa is observed)

Aufreinigung: serum

Immunogen: psTOC75, Predicted POTRA Domain #1, Amino acids, 158-241, Expressed and purified in E. coli using the Impact System from NEB. Peptide confirmed by MALDI. Q43715

Anwendungsinformationen

Applikationshinweise: 1: 2000 - 1: 25 000, 1: 100 000 (WB), 1: 500 (FACS). 1: 100 (IL)

Kommentare:

antibody detects Toc75 POTRA domain 1 as purified protein, in chloroplast fraction and in crude envelope fraction

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 200 μL of sterile water

Handhabung: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Details zu Toc75

Target: Toc75 (Chloroplast Outer Envelope Membrane Translocon Complex Oep75 Protein (Toc75))

Hintergrund: OEP 75 or Toc75, Chloroplast outer envelope membrane protein from Pisum sativum (pea), Predicted to contain 3 POTRA domains at N-terminus. Believed to be the protein conducting channel of the Toc translocon and assembles as an 18 stranded ß-barrel (EMBO J. (1995) 14:11, 2436-2446), In Arabidopsis there are five members of this Family Toc75 (I-V), atToc75III is most closely related to psToc75. Additionally, it is structurally related to members of the bacterial surface antigen super-family including: OMA87, Outer membrane protein/protective antigen, (COG4775, COG4775) [Cell envelope biogenesis, outer membrane, YaeT, outer membrane protein assembly complex, (TIGR03303), FhaC, Hemolysin activation/secretion protein (COG2831) [Intracellular trafficking and secretion]

Molekulargewicht: expected: 88 kDa, apparent: 75 kDa (ocassionally a processing intermediate at 78 kDa is observed)

Gen-ID: 158241

UniProt: Q43715