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SELPLG Antikörper (FITC)

SELPLG Reaktivität: Human FACS Wirt: Maus Monoclonal PL2 FITC
Produktnummer ABIN488144
  • Target Alle SELPLG Antikörper anzeigen
    SELPLG (Selectin P Ligand (SELPLG))
    Reaktivität
    • 77
    • 36
    • 21
    • 1
    • 1
    • 1
    Human
    Wirt
    • 68
    • 30
    • 15
    Maus
    Klonalität
    • 66
    • 46
    • 1
    Monoklonal
    Konjugat
    • 43
    • 8
    • 6
    • 6
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser SELPLG Antikörper ist konjugiert mit FITC
    Applikation
    • 74
    • 72
    • 25
    • 19
    • 16
    • 9
    • 8
    • 7
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Spezifität
    This antibody reacts with Human PSGL-1 antigen.
    Kreuzreaktivität (Details)
    Species reactivity (tested):Human.
    Aufreinigung
    Protein-A Sepharose Chromatography.
    Immunogen
    Human Neutrophil PSGL-1. Remarks: This Hybridoma was established by fusion of mouse myeloma cell P3-X63-Ag8-653 with Balb/c mouse splenocyte.
    Klon
    PL2
    Isotyp
    IgG1
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  • Applikationshinweise
    Flow Cytometry: 10-20 μg/mL (final concentration). Positive Controls: Jurkat, HL60.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Protokoll
    Flow Cytometric Analysis for Floating CellsProtocol 1We use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and0. 1% NaN3]. 2) Add 10 µL of normal goat serum to the cell pellet aftertapping. Mix well, and incubate for 5 minutes at roomtemperature (20~25°C). 3) Add 30 µL of the FITC labeled CD162 (PL2) monoclonal antibody (10-20 µg/mL) dilutedwith the washing buffer. Mix well, and incubate for 30 minutes at room temperature(20~25°C). 4) Add 1 mL of the washing buffer followed by centrifugation at 500xg for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 5) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. Protocol 2We usually use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and0. 1% NaN3]. 2) Resuspend the cells with PBS containing 25% normal goat serum, 1 mg/mL normalhuman IgG and 0. 1% NaN3 (5x10e6 cells/mL). 3) Add 20 µL of the non diluted FITC labeled CD162 (PL1) monoclonal antibody (50 µg/mL)into each tube. 4) Add 50 µL of the cell suspension into each tube. Mix well, and incubate for 30 minutesat room temperature (20~25°C)5) Add 1 mL of the washing buffer followed by centrifugation at 500xg for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 6) Resuspend the cells with 500 μL of the washing buffer and analyze by a flow cytometer.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.05 mg/mL
    Buffer
    PBS, pH 7.2 containing 1 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store the antibody undiluted at 2-8 °C.
  • Target
    SELPLG (Selectin P Ligand (SELPLG))
    Andere Bezeichnung
    CD162 / PSGL1 (SELPLG Produkte)
    Synonyme
    CD162 antikoerper, CLA antikoerper, PSGL-1 antikoerper, PSGL1 antikoerper, SELPLG antikoerper, SELPG antikoerper, Psgl-1 antikoerper, Psgl1 antikoerper, Selp1 antikoerper, Selpl antikoerper, selectin P ligand antikoerper, selectin, platelet (p-selectin) ligand antikoerper, clasper antikoerper, SELPLG antikoerper, Selplg antikoerper, cla antikoerper
    Hintergrund
    CD162 is a 120kD molecule also known as P-Selectin Glycoprotein Ligand I (PSGL-1). CD162 is the major counter receptor for P-selectin and is involved in neutrophil attachment and rolling to endothelium. In peripheral blood CD162 is expressed by monocytes, granulocytes, T cells and some B cells.Synonyms: P-selectin glycoprotein ligand 1, PSGL-1, SELPLG, Selectin P ligand
    Gen-ID
    6404
    NCBI Accession
    NP_002997
    UniProt
    Q14242
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