Methotrexate Antikörper

Produktnummer ABIN487772

Produktdetails anti-Methotrexate Antikörper

Target: Methotrexate (MTX)

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser Methotrexate Antikörper ist unkonjugiert

Applikation: Enzyme Immunoassay (EIA)

Aufreinigung: Ig Fraction

Immunogen: Methotrexate -BSA

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 2.5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 7.74 mg/mL (U.V.abs at 280nm)

Buffer: 20 mM Phosphate, 150 mM Sodium Chloride, pH 7.2 containing 0.09 % Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu Methotrexate

Target: Methotrexate (MTX)

Andere Bezeichnung: Methotrexate (MTX Produkte)

Substanzklasse: Amino Acid

Hintergrund: Methotrexate (rINN) abbreviated MTX and formerly known as amethopterin, is an antimetabolite and antifolate drug used in treatment of cancer, autoimmune diseases and as an abortifacient in the induction of medical abortions. It acts by inhibiting the metabolism of folic acid. Methotrexate competitively inhibits dihydrofolate reductase (DHFR), an enzyme that participates in the tetrahydrofolate synthesis. The affinity of methotrexate for DHFR is about one thousand-fold that of folate for DHFR. Methotrexate acts specifically during DNA and RNA synthesis, and thus it is cytotoxic during the S-phase of the cell cycle. Logically, it therefore has a greater toxic effect on rapidly dividing cells which replicate their DNA more frequently, and thus inhibits the growth and proliferation of these non-cancerous cells. Lower doses of methotrexate have been shown to be very effective for the management of rheumatoid arthritis, Crohn's disease, and psoriasis.Synonyms: 4-amino-10-methylpteroyl-L-glutamic acid