B-Cells Antikörper

Produktnummer ABIN481701

Der Maus Monoklonal anti-B-Cells Antikörper (Klon Ki-B1R) (ABIN481701) detektiert spezifisch B-Cells in IHC und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Ratte.

Kurzübersicht für B-Cells Antikörper (ABIN481701)

Target: B-Cells

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: Ki-B1R

Produktdetails

Spezifität: Ki-B1R recognizes a surface antigen of 47  kDa molecular weight, which appears as a single band under reducing conditions in SDS-PAGE. The epitope has not been further characterized. Antigen distribution: Isolated cells: In a test comparing the immunophenotype of rat blood lymphocytes, Ki-B1R recognised 19 % + 3 % of peripheral blood lymphocytes (n=5). In the same test, 18 % +/- 3 % of the lymphocytes stained positive for sIg, whereas 79 % +/- 3 % stained positive with Ki-T1R, the rat pan T-cell marker. Tissue sections: Ki-B1R shows a staining pattern similar to that of anti-rat immunoglobulin markers. Follicular mantle B- lymphocytes are strongly positive whereas staining of germinal centre B-lymphocytes depends on the immunological status of the lyphoid organ. In the gastrointestinal tract, the typical mucosa-associated lymphoid tissue (MALT) selectively displays the B-follicles, mostly those containing prominent germinal centres. The reactivity pattern of Ki-B1R does not change when applied in vivo. Specificity: Rat: mature B-lymphocytes, plasma cells. Other species: not tested.

Aufreinigung: Affinity purified

Immunogen: Lymphocytes

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (1:100)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.5 mL distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS  pH 7.2, 5 mg/mL BSA, 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid freeze/thaw cycles. Store undiluted.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.

Antigendetails

Target: B-Cells

Andere Bezeichnung: B Cell