S100A8/A9 Complex (Calprotectin) Antikörper

Produktnummer ABIN481651

Dieser Maus Monoklonal Antikörper detektiert spezifisch S100A8/A9 Complex (Calprotectin) in IHC, ELISA und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für S100A8/A9 Complex (Calprotectin) Antikörper (ABIN481651)

Target: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser S100A8/A9 Complex (Calprotectin) Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: 27E10

Produktdetails anti-S100A8/A9 Complex (Calprotectin) Antikörper

Spezifität: The antigen is MRP8/14 (calprotectin), the epitope involves parts of both subunits MRP8 and MRP14. Antigen distribution: Isolated cells: Monocytes carry the antigen both on the surface and intracellularly, granulocytes exhibit it only intracellularly. Up to 80 % of monocytes in early cultures (24- 48h) are positive. No reaction has been seen with lymphocytes or platelets. Tissue sections: The antigen is found in macrophages in the red pulp of the spleen and liver. It is strongly expressed in macrophages from acute inflamed tissues (peridontitis, contact eczema, urticaria, erythrodermia) where some endothelial and epidermal cells may also express this antigen. It is normally absent on resident mononuclear phagocytes in healthy tissues (skin, gut, thymus). Specificity: Human: subpopulation of macrophages, monocytes and granulocytes, peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. Other: subpopulation of macrophages of rhesus monkey and bovine tissues. The antibody does not react with swine tissues.

Aufreinigung: Affinity purified

Immunogen: Cultured human monocytes

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IHC, IHC-P (1 μg/mL)

Usage: For IHC-P, Proteinase K pretreatment is recommended for antigen retrieval.

Not recommended for: IHC-Fr

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled Water.

Konzentration: Lot specific

Buffer: Lyophilized from PBS  pH 7.2, 5 mg/mL BSA, and 0.05 % Kathon CG

Konservierungsmittel: Kathon CG

Handhabung: Avoid freeze/thaw cycles. Store undiluted.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.

Details zu S100A8/A9 Complex (Calprotectin)

Target: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

Andere Bezeichnung: Calprotectin

Pathways: S100 Proteine