IFNGR1 Antikörper (Extracellular Domain)

Produktnummer ABIN480396

Dieses Anti-IFNGR1-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von IFNGR1 in WB, IHC, ELISA, ICC, Neut und Func. Geeignet für Human.

Kurzübersicht für IFNGR1 Antikörper (Extracellular Domain) (ABIN480396)

Target: IFNGR1 (Interferon gamma Receptor 1 (IFNGR1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser IFNGR1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Neutralization (Neut), Functional Studies (Func)

Produktdetails anti-IFNGR1 Antikörper

Bindungsspezifität: Extracellular Domain

Spezifität: Binds to and neutralizes human Interferon gamma receptor 1, 15.756kD. Blocks cell surface human Interferon gamma receptor 1-mediated bioactivity of recombinant human Interferon gamma receptor 1. In direct ELISA and Western Blot, does not cross-react with human Interferon gamma receptor chain2.

Aufreinigung: Immunoaffinity purified

Immunogen: Recombinant, human Interferon gamma receptor chain 1, NS0-derived, extracellular domain.

Type of Immunogen: Recombinant protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, Func, ICC, IHC (15 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Neutralization. Neutralization of Human IFN-g R1 Bioactivity: The exact concentration of antibody required to neutralize rhIFN-g R1-mediated bioactivity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. The ND50 is ~0.5-2 μg/mL in the presence of 2 ng/mL of rhIFN-g, using a confluent culture of HeLa cells. ELISA (Direct): 0.5-1 μg/mL. The detection limit for rhIFN-g R1 is ~0.3 ng/well. Western Blot: 0.1-0.2 μg/mL. The detection limit for is ~5 ng/lane under non-reducing and reducing conditions. Immunohistochemistry: 15 μg/mL. Detects IFN-g R1 in human PBMC's (peripheral blood mononuclear cells) (fluorescence immunocytochemistry).

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 5 % trehalose. No preservative added.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu IFNGR1

Target: IFNGR1 (Interferon gamma Receptor 1 (IFNGR1))

Andere Bezeichnung: CD119 / IFNGR1

Hintergrund: Name/Gene ID: IFNGR1
Family: Interferon Receptor

Synonyms: IFNGR1, AVP, type 2, Antiviral protein, type 2, CD119, CD119 antigen, IFNGR, IFN-gamma receptor 1, IFN-gamma-R1, Immune interferon receptor 1, Interferon gamma receptor 1, CDw119

Gen-ID: 3459

UniProt: P15260

Pathways: Interferon-gamma Pathway