SSEA-4 Antikörper
Kurzübersicht für SSEA-4 Antikörper (ABIN480275)
Target
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
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Spezifität
- Recognizes a carbohydrate epitope of human SSEA-4. Species cross-reactivity: Mouse.
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Aufreinigung
- Protein A purified
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Immunogen
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Human embryonal carcinoma cell line 2102 Ep.
Type of Immunogen: Cells -
Isotyp
- IgG3
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Applikationshinweise
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Approved: Flo, IHC, IHC-Fr
Usage: Flow Cytometry, Immunohistochemistry. Immunohistochemistry: This antibody has been used with the appropriate secondary reagents at a concentration of 5-10 μg/mL in fixed cells. Cells were fixed with 4 % paraformaldehyde in PBS at room temperature for 20 minutes, then blocked with PBS containing 10 % normal donkey serum and 1 % BSA at room temperature for 45 minutes. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine Red coupled anti-mouse IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. This antibody can also be used in unfixed, shock frozen tissue at a concentration of 10 μg/mL. Flow Cytometry: Dilute this antibody to 0.1 mg/mL and add 5 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. -
Kommentare
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Target Species of Antibody: Human
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- Reconstitution: Reconstitute, sterile PBS. If 0.2 mL of PBS is used, the antibody concentration will be 500 μg/mL.
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Konzentration
- Lot specific
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Buffer
- Lyophilized from TBS, 5 % trehalose. Reconstitution: Reconstitute, sterile PBS. If 0.2 mL of PBS is used, the antibody concentration will be 500 μg/mL.
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- SSEA-4 (Thiosulfate Sulfurtransferase 4 (SSEA-4))
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Andere Bezeichnung
- SSEA-4
Target
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