Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

SSEA-4 Antikörper

Der Maus Monoklonal Anti-SSEA-4-Antikörper wurde für FACS, IHC und IHC (fro) validiert. Er ist geeignet, SSEA-4 in Proben von Human und Maus zu detektieren.
Produktnummer ABIN480275

Kurzübersicht für SSEA-4 Antikörper (ABIN480275)

Target

SSEA-4 (Thiosulfate Sulfurtransferase 4 (SSEA-4))

Reaktivität

Human, Maus

Wirt

  • 13
Maus

Klonalität

  • 13
Monoklonal

Konjugat

  • 4
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser SSEA-4 Antikörper ist unkonjugiert

Applikation

  • 12
  • 4
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • Spezifität

    Recognizes a carbohydrate epitope of human SSEA-4. Species cross-reactivity: Mouse.

    Aufreinigung

    Protein A purified

    Immunogen

    Human embryonal carcinoma cell line 2102 Ep.

    Type of Immunogen: Cells

    Isotyp

    IgG3
  • Applikationshinweise

    Approved: Flo, IHC, IHC-Fr

    Usage: Flow Cytometry, Immunohistochemistry. Immunohistochemistry: This antibody has been used with the appropriate secondary reagents at a concentration of 5-10 μg/mL in fixed cells. Cells were fixed with 4 % paraformaldehyde in PBS at room temperature for 20 minutes, then blocked with PBS containing 10 % normal donkey serum and 1 % BSA at room temperature for 45 minutes. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine Red coupled anti-mouse IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. This antibody can also be used in unfixed, shock frozen tissue at a concentration of 10 μg/mL. Flow Cytometry: Dilute this antibody to 0.1 mg/mL and add 5 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome.

    Kommentare

    Target Species of Antibody: Human

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Reconstitution: Reconstitute, sterile PBS. If 0.2 mL of PBS is used, the antibody concentration will be 500 μg/mL.

    Konzentration

    Lot specific

    Buffer

    Lyophilized from TBS, 5 % trehalose. Reconstitution: Reconstitute, sterile PBS. If 0.2 mL of PBS is used, the antibody concentration will be 500 μg/mL.
  • Target

    SSEA-4 (Thiosulfate Sulfurtransferase 4 (SSEA-4))

    Andere Bezeichnung

    SSEA-4
Sie sind hier:
Chat with us!