Neurofilament 150, 200kD Antikörper

Produktnummer ABIN479504

Produktdetails

Target: Neurofilament 150, 200kD

Reaktivität: Human, Maus, Kaninchen, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: Recognizes the 160 alpha 200kD proteins of human neurofilament. Specifically recognizes a non-phosphorylated epitope in the tail domain of NF-M+H. Dephosphorylation of NF-M+H will increase immunoreactivity with this antibody. Species cross-reactivity: human, rat, mouse, bovine, rabbit, hamster and squid.

Aufreinigung: Purified

Immunogen: Adult rat neurofilament.

Type of Immunogen: Protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.1 - 0.5 μg/mL), IHC, IHC-Fr, IHC-P, IP, WB (0.5 - 1 μg/mL)

Usage: Suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation, and Western Blot. ELISA: 0.1-0.5 μg/mL. Immunohistochemistry (Bouin's and alcohol-fixed, paraffin-embedded sections and frozen sections): 5-10 μg/mL. To stain, incubate 30-60 minutes at RT or overnight at 4?C. Western Blot: 0.5-1 μg/mL. Immunoprecipitation: 2-5 μg.

Kommentare:

Target Species of Antibody: Rat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Antigendetails

Target: Neurofilament 150, 200kD

Andere Bezeichnung: Neurofilament 150, 200kD (NEFM/NEFH)

Hintergrund: This antibody detects: NEFM + NEFH