Proliferation Marker Antikörper

Produktnummer ABIN478915

Produktdetails

Target: Proliferation Marker

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Spezifität: Reacts with a 14-16kD protein as demonstrated by western blot analysis on the Raji cell line. This antigen was detected after 12 h of PHA-induced activation in the early G1 phase but absent in non-stimulated lymphocytes. Cross reactivity is likely to occur with mouse and rat.

Aufreinigung: Protein A purified

Immunogen: Spleen cells of a human patient with hairy cell leukemia.

Type of Immunogen: Cells

Isotyp: IgM kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:1000), IHC, IHC-Fr, IHC-P, IP, WB (1:50)

Usage: Suitable for use in ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry frozen and formalin/paraffin). The antibody is reported to recognize a nuclear antigen that is present in the cytoplasm and nuclei of proliferating cells paraformaldehyde fixed, Triton X-100 permeabilized). An increase of 400 % is observed in cells in mitosis (determined by analysis of K562 cells synchronized with colcemide). IPO-38 does not block the binding of the Ki-67 antibody. ELISA: 1:1000. Immunohistochemistry:1:50 to 1:100. Western Blot: 1:50 to 1:100. Positive control: Tonsilar tissue.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.2, 0.01 % sodium azide. No stabilizing proteins added.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Antigendetails

Target: Proliferation Marker