Fibroblasts Antikörper
Kurzübersicht für Fibroblasts Antikörper (ABIN472810)
Target
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
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Spezifität
- Recognizes human fibroblasts in tissue as well as cultured fibroblasts. On skin sections the antibody reacts with stratum basal epidermal cells. The antigen appears to be a marker for mesodermally dervied (mesenchymal) human connective tissue and tumors derived from such tissue (e.g. fibrosarcomas) {Haynes et al, 1984}. Useful for the assessment of thymic epithelial culture contamination with fibroblasts, and the identification of cells of mesodermal origin in the fetus.
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Aufreinigung
- Ion exchange chromatography
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Immunogen
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Whole human thymic stoma cells.
Type of Immunogen: Cells -
Isotyp
- IgG1
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Applikationshinweise
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Approved: Flo (1:100), IF (1:100 - 1:200)
Usage: Suitable for use in Immunofluorescence, FACS, and the assessment of thymic epithelial culture contamination with fibroblasts and the identification of cells of mesodermal origin in the fetus. Immunofluorescence: 1:100-1:200 (acetone fixed tissue). FACS: 1:100 (fresh or acetone fixed tissue, do not use trypsin to prepare adherent cell lines, use a non-enzymatic detachment procedure. -
Kommentare
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Target Species of Antibody: Human
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- Lot specific
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Buffer
- PBS, 10 mM sodium azide, 1 mg/mL BSA.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- avoid freeze thaw cycles.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- Fibroblasts
Target
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