Cyclin D1/D2 Antikörper

Produktnummer ABIN470767

Dieses Maus Monoklonal-Antikörper erkennt spezifisch in IP, WB, IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Hamster, Maus und Ratte.

Kurzübersicht für Cyclin D1/D2 Antikörper (ABIN470767)

Target: Cyclin D1/D2

Reaktivität: Human, Hamster, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails

Spezifität: Recognizes p36 cyclin D1 and cross-reacts with p34 cyclin D2, which has a highly homologous epitope. No cross-reactivity with cyclin A, cyclin B or cyclin D3. Species cross-reactivity: human, mouse, rat and hamster.

Aufreinigung: Protein A purified

Immunogen: Recombinant human Cyclin D1 expressed in E. coli.

Type of Immunogen: Recombinant protein

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P, IP, WB

Usage: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 5 μg immunoprecipitates cyclin D1 and D2 from 500 μg of A431 RIPA lysate. Immunohistochemistry: Reported to detect cyclin D1 and D2 in paraffin-embedded sections using high energy antigen retrieval (10 mM citrate buffer,  pH 6.0, microwaved 3-5 minutes, three times).

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.2, 50 % glycerol.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Antigendetails

Target: Cyclin D1/D2

Andere Bezeichnung: Cyclin D1,2

Hintergrund: This antibody detects: Cyclin D1+D2