Cyclin D1/D2 Antikörper
Reaktivität: Human, Hamster, Maus, Ratte
IP, WB, IHC, IHC (p)
Wirt: Maus
Monoclonal
unconjugated
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- Target
- Cyclin D1/D2
- Reaktivität
- Human, Hamster, Maus, Ratte
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Unkonjugiert
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Applikation
- Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Spezifität
- Recognizes p36 cyclin D1 and cross-reacts with p34 cyclin D2, which has a highly homologous epitope. No cross-reactivity with cyclin A, cyclin B or cyclin D3. Species cross-reactivity: human, mouse, rat and hamster.
- Aufreinigung
- Protein A purified
- Immunogen
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Recombinant human Cyclin D1 expressed in E. coli.
Type of Immunogen: Recombinant protein - Isotyp
- IgG2a
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- Applikationshinweise
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Approved: IHC, IHC-P, IP, WB
Usage: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 5 μg immunoprecipitates cyclin D1 and D2 from 500 μg of A431 RIPA lysate. Immunohistochemistry: Reported to detect cyclin D1 and D2 in paraffin-embedded sections using high energy antigen retrieval (10 mM citrate buffer, pH 6.0, microwaved 3-5 minutes, three times). - Kommentare
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Target Species of Antibody: Human
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- Lot specific
- Buffer
- PBS, pH 7.2, 50 % glycerol.
- Handhabung
- Avoid repeat freeze-thaw cycles.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- Cyclin D1/D2
- Andere Bezeichnung
- Cyclin D1,2
- Hintergrund
- This antibody detects: Cyclin D1+D2
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