GRO gamma Antikörper

Produktnummer ABIN465909

Der Kaninchen Polyklonal Anti-GRO gamma-Antikörper wurde für WB, ELISA und Neut validiert. Er ist geeignet, GRO gamma in Proben von Human zu detektieren.

Kurzübersicht für GRO gamma Antikörper (ABIN465909)

Target: GRO gamma (Growth Regulated Oncogene gamma (GRO gamma))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GRO gamma Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Produktdetails anti-GRO gamma Antikörper

Spezifität: Human GROgamma

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human GROgamma.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 2 μg/mL), Neut (1 - 2 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.7 mg/mL-0.5 mg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human GRO-gamma (100 ng/mL), a concentration of 1-2 μg/mL of this antibody is required. ELISA: To detect Human GRO-gamma by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human GRO-gamma (LS-C104834) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human GRO-gamma. Western Blot: To detect Human GRO-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human GRO-gamma is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with sterile water

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu GRO gamma

Target: GRO gamma (Growth Regulated Oncogene gamma (GRO gamma))

Andere Bezeichnung: CXCL3 / GRO Gamma

Hintergrund: Name/Gene ID: CXCL3
Subfamily: Intercrine alpha
Family: Intercrine

Synonyms: CXCL3, C-X-C motif chemokine 3, GRO3 oncogene, Growth-regulated protein gamma, GRO3, GRO-gamma, GRO-gamma(1-73), MGSA gamma, MIP2B, MIP-2b, MIP2-beta, CINC-2b, GROg, SCYB3, Small inducible cytokine b3

Gen-ID: 2921

UniProt: P19876