CXCL9 Antikörper

Produktnummer ABIN465877

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch CXCL9 in WB, ELISA und Neut. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für CXCL9 Antikörper (ABIN465877)

Target: CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CXCL9 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Produktdetails anti-CXCL9 Antikörper

Spezifität: Human MIG

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human MIG.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 2 μg/mL), Neut (5 - 7 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human renal tumor with parenchyma tissue. The recommended concentration is 0.25 μg/mL-0.50 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved with heat induced antigen retrieval with a  pH 6 sodium citrate buffer. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hMIG (100 ng/mL), a concentration of 5-7 μg/mL of this antibody is required. ELISA: To detect hMIG by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MIG (LS-C104825) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMIG. Western Blot: To detect hMIG by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIG is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile water

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu CXCL9

Target: CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))

Andere Bezeichnung: MIG / CXCL9

Hintergrund: Name/Gene ID: CXCL9
Subfamily: Intercrine alpha
Family: Intercrine

Synonyms: CXCL9, Crg-10, Humig, SCYB9, Small-inducible cytokine B9, C-X-C motif chemokine 9, CMK, MIG

Gen-ID: 4283

UniProt: Q07325