Ccl12 Antikörper (Biotin)

Produktnummer ABIN465850

Dieses Anti-Ccl12-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Ccl12 in ELISA, WB und Neut. Geeignet für Maus.

Kurzübersicht für Ccl12 Antikörper (Biotin) (ABIN465850)

Target: Ccl12 (Chemokine (C-C Motif) Ligand 12 (Ccl12))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Ccl12 Antikörper ist konjugiert mit Biotin

Applikation: ELISA, Western Blotting (WB), Neutralization (Neut)

Produktdetails anti-Ccl12 Antikörper (Biotin)

Spezifität: Mouse MCP-5

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Murine MCP-5.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect mMCP-5 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mMCP-5. To detect mMCP-5 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine MCP-5 (LS-C104804) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mMCP-5. Western Blot: To detect mMCP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-5 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile PBS containing 0.1 % BSA

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu Ccl12

Target: Ccl12 (Chemokine (C-C Motif) Ligand 12 (Ccl12))

Andere Bezeichnung: CCL12 / MCP5

Hintergrund: Name/Gene ID: Ccl12

Synonyms: Ccl12, MCP-5, MCP5, Scya12

Gen-ID: 20293

UniProt: Q62401

Pathways: Cellular Response to Molecule of Bacterial Origin