Nerve Growth Factor Antikörper

Produktnummer ABIN465644

Der Kaninchen Polyklonal anti-Nerve Growth Factor Antikörper wird verwendet zum Nachweis von Nerve Growth Factor in Proben von Human. Er wurde validiert für WB, ELISA und Neut.

Kurzübersicht für Nerve Growth Factor Antikörper (ABIN465644)

Target: Nerve Growth Factor (NGF)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Nerve Growth Factor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Produktdetails anti-Nerve Growth Factor Antikörper

Spezifität: Human beta-NGF

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human beta-NGF.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 2 μg/mL), Neut (5 - 8 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human pancreas adenocarcinoma. The recommended concentration is 0.5 mg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results were achieved without antigen retrieval. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human beta-NGF (1 ng/mL), a concentration of 0.05-0.08 μg/mL of this antibody is required. ELISA: To detect Human beta-NGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human beta-NGF (LS-C104735) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human beta-NGF. Western Blot: To detect Human beta-NGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human beta-NGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile water

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu Nerve Growth Factor

Target: Nerve Growth Factor (NGF)

Andere Bezeichnung: NGF

Hintergrund: Name/Gene ID: NGF
Family: NGF Beta

Synonyms: NGF, Beta-nerve growth factor, ProNGF, Beta-NGF, HSAN5, NGFB

Gen-ID: 4803

UniProt: P01138

Pathways: Regulation of Cell Size