TNFSF13 Antikörper

Produktnummer ABIN465417

Der Kaninchen Polyklonal anti-TNFSF13 Antikörper (ABIN465417) detektiert spezifisch TNFSF13 in WB und ELISA. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für TNFSF13 Antikörper (ABIN465417)

Target: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TNFSF13 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-TNFSF13 Antikörper

Spezifität: Human APRIL

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human APRIL.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human carcinoid tissue. The recommended concentration is 0.25 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary. ELISA: To detect hAPRIL by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human APRIL (LS-C104484) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hAPRIL. Western Blot: To detect hAPRIL by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hAPRIL is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile water

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu TNFSF13

Target: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Andere Bezeichnung: TNFSF13 / APRIL

Hintergrund: Name/Gene ID: TNFSF13
Family: TNF

Synonyms: TNFSF13, APRIL, CD256, CD256 antigen, TALL-2, TALL2, ZTNF2, TNF-related death ligand 1, TRDL-1, UNQ383/PRO715

Gen-ID: 8741

UniProt: O75888

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response