AIMP1 / EMAP II Antikörper (Biotin)
-
- Target
- AIMP1 / EMAP II
-
Reaktivität
- Human
-
Wirt
- Ziege
-
Klonalität
- Polyklonal
-
Konjugat
- Biotin
-
Applikation
- Western Blotting (WB), ELISA
- Spezifität
- Human EMAP-II
- Aufreinigung
- Immunoaffinity purified
- Immunogen
-
E.coli derived recombinant Human EMAP-II.
Type of Immunogen: Cell extract
-
-
- Applikationshinweise
-
Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hEMAP-II by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hEMAP-II. To detect hEMAP-II by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human EMAP-II (LS-C104512) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hEMAP-II. Western Blot: To detect hEMAP-II by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEMAP-II is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - Kommentare
-
Target Species of Antibody: Human
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Lyophilized
- Rekonstitution
- Reconstitute in sterile PBS with 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
- Konzentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.2
- Handhabung
- Avoid freeze/thaw cycles.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
-
Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
-
- Target
- AIMP1 / EMAP II
- Hintergrund
-
Name/Gene ID: AIMP1
Synonyms: AIMP1, EMAP II, EMAPII, EMAP-2, HLD3, p43, EMAP2, SCYE1 - Gen-ID
- 9255
- UniProt
- Q12904
-