AIMP1 / EMAP II Antikörper (Biotin)

Produktnummer ABIN465270

Der Ziege Polyklonal anti-AIMP1 / EMAP II Antikörper wird verwendet zum Nachweis von AIMP1 / EMAP II in Proben von Human. Er wurde validiert für WB und ELISA.

Kurzübersicht für AIMP1 / EMAP II Antikörper (Biotin) (ABIN465270)

Target: AIMP1 / EMAP II

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Biotin

Applikation: Western Blotting (WB), ELISA

Produktdetails

Spezifität: Human EMAP-II

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human EMAP-II.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hEMAP-II by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hEMAP-II. To detect hEMAP-II by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human EMAP-II (LS-C104512) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hEMAP-II. Western Blot: To detect hEMAP-II by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEMAP-II is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute in sterile PBS with 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Antigendetails

Target: AIMP1 / EMAP II

Hintergrund: Name/Gene ID: AIMP1

Synonyms: AIMP1, EMAP II, EMAPII, EMAP-2, HLD3, p43, EMAP2, SCYE1

Gen-ID: 9255

UniProt: Q12904