LGALS1/Galectin 1 Antikörper (Biotin)

Produktnummer ABIN465025

Der Biotin-konjugierte Kaninchen Polyklonal anti-LGALS1/Galectin 1 Antikörper (ABIN465025) detektiert spezifisch LGALS1/Galectin 1 in WB und ELISA. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für LGALS1/Galectin 1 Antikörper (Biotin) (ABIN465025)

Target: LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LGALS1/Galectin 1 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-LGALS1/Galectin 1 Antikörper (Biotin)

Spezifität: Human Galectin-1

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human Galectin-1.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: ELISA: To detect hGalectin-1 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hGalectin-1. To detect hGalectin-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human Galectin-1 (LS-B7436) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hGalectin-1. Western Blot: To detect hGalectin-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGalectin-1 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile PBS containing 0.1 % BSA

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu LGALS1/Galectin 1

Target: LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

Andere Bezeichnung: LGALS1 / Galectin 1

Hintergrund: Name/Gene ID: LGALS1

Synonyms: LGALS1, 14 kDa lectin, 14 kDa laminin-binding protein, 14 kd lectin, GAL1, Galectin 1, Galectin-1, HBL, Lactose-binding lectin 1, Galaptin, GBP, HLBP14, HPL, S-Lac lectin 1, Gal-1

Gen-ID: 3956

UniProt: P09382

Pathways: Carbohydrate Homeostasis