LTA Antikörper (Biotin)

Produktnummer ABIN465016

Dieser Anti-LTA Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von LTA in WB, ELISA und Neut. Geeignet für Human.

Kurzübersicht für LTA Antikörper (Biotin) (ABIN465016)

Target: LTA (Lymphotoxin-alpha (LTA))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LTA Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Produktdetails anti-LTA Antikörper (Biotin)

Spezifität: Human TNF-beta

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human TNF-beta.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect Human TNF-beta by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human TNF-beta. To detect Human TNF-beta by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human TNF-beta (LS-C104710) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human TNF-beta. Western Blot: To detect Human TNF-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human TNF-beta is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile PBS containing 0.1 % BSA

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu LTA

Target: LTA (Lymphotoxin-alpha (LTA))

Andere Bezeichnung: LTA / TNF Beta

Substanzklasse: Chemical

Hintergrund: Name/Gene ID: LTA
Family: TNF

Synonyms: LTA, Lymphotoxin-alpha, LT-alpha, TNFSF1, TNF-beta, TNFB, Tumor necrosis factor beta, LT, TNF Beta

Gen-ID: 4049

UniProt: P01374

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process