CXCL10 Antikörper

Produktnummer ABIN465012

Der Kaninchen Polyklonal anti-CXCL10 Antikörper wird verwendet zum Nachweis von CXCL10 in Proben von Human. Er wurde validiert für WB, ELISA und Neut.

Kurzübersicht für CXCL10 Antikörper (ABIN465012)

Target: CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CXCL10 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Produktdetails anti-CXCL10 Antikörper

Spezifität: Human IP-10

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human IP-10.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 2 μg/mL), Neut (2 - 3 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human carcinoid tissue. The recommended concentration is 1 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IP-10 (100 ng/mL), a concentration of 5-8 μg/mL of this antibody is required. ELISA: To detect hIP-10 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IP-10 (LS-C104616) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIP-10. Western Blot: To detect hIP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIP-10 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile water

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu CXCL10

Target: CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))

Andere Bezeichnung: IP-10 / CXCL10

Hintergrund: Name/Gene ID: CXCL10
Subfamily: Intercrine alpha
Family: Intercrine

Synonyms: CXCL10, C-X-C motif chemokine 10, Gamma-IP10, IP-10, Gamma IP10, GIP-10, Mob-1, Small-inducible cytokine B10, Crg-2, IFI10, INP10, SCYB10

Gen-ID: 3627

UniProt: P02778