MIA Antikörper

Produktnummer ABIN464851

Produktdetails anti-MIA Antikörper

Target: MIA (Melanoma Inhibitory Activity (MIA))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MIA Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB)

Spezifität: Human MIA

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human MIA.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human pancreas infiltrating ductal adenocarcinoma tissue. The recommended concentration is 1 μg/mL-2 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Optimal results for these conditions were achieved with heat induced antigen retrieval with a  pH 6 Sodium Citrate buffer or enzyme induced antigen retrieval with proteinase K at room temperature. Optimal concentrations and conditions may vary. ELISA: To detect hMIA by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MIA (LS-C104284) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMIA. Western Blot: To detect hMIA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIA is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu MIA

Target: MIA (Melanoma Inhibitory Activity (MIA))

Andere Bezeichnung: MIA (MIA Produkte)

Synonyme: CD-RAP antikoerper, Mia1 antikoerper, Cdrap antikoerper, zgc:194157 antikoerper, mia antikoerper, CDRAP antikoerper, Fdp antikoerper, MIA antikoerper, MIAL antikoerper, melanoma inhibitory activity antikoerper, otoraplin antikoerper, cilia and flagella associated protein 100 antikoerper, MIA antikoerper, Mia antikoerper, mia antikoerper, Otor antikoerper, CFAP100 antikoerper

Hintergrund: Name/Gene ID: MIA
Family: MIA

Synonyms: MIA, CD-RAP, Melanoma inhibitory activity

Gen-ID: 8190

UniProt: Q16674