CCL15 Antikörper

Produktnummer ABIN464833

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch CCL15 in ELISA, WB und Neut. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CCL15 Antikörper (ABIN464833)

Target: CCL15 (Chemokine (C-C Motif) Ligand 15 (CCL15))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CCL15 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Neutralization (Neut)

Produktdetails anti-CCL15 Antikörper

Spezifität: Human MIP-5

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human MIP-5.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 2 μg/mL), Neut (1.75 - 3 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.5 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hMIP-5 (50 ng/mL), a concentration of 1.75-3 μg/mL of this antibody is required. ELISA: To detect hMIP-5 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MIP-5 (LS-C104743) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMIP-5. Western Blot: To detect hMIP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-5 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile water

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu CCL15

Target: CCL15 (Chemokine (C-C Motif) Ligand 15 (CCL15))

Andere Bezeichnung: CCL15 / MIP5

Hintergrund: Name/Gene ID: CCL15
Subfamily: Intercrine beta
Family: Intercrine

Synonyms: CCL15, C-C motif chemokine 15, Chemokine hcc2, HMRP-2B, Leukotactin-1, LKN1, MIP-5, MIP5, MRP-2B, Leukotactin1, Small-inducible cytokine A15, MIP-1D, NCC3, New CC chemokine 3, SY15, Chemokine CC-2, HCC-2, Leukotactin 1, LKN-1, MIP-1 delta, NCC-3, SCYA15

Gen-ID: 6359

UniProt: Q16663