ALPL Antikörper

Produktnummer ABIN462352

Dieses Maus Monoklonal-Antikörper erkennt spezifisch ALPL in WB, ELISA, IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human und Rind (Kuh).

Kurzübersicht für ALPL Antikörper (ABIN462352)

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Reaktivität: Human, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ALPL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-ALPL Antikörper

Marke: IHC-plus™

Spezifität: Recognizes Alkaline Phosphatase from human bone. Species cross-reactivity: Alkaline phosphatase from human placenta and calf intestine.

Aufreinigung: Protein A purified

Immunogen: Purified alkaline phosphatase (native) from human bone.

Type of Immunogen: Purified protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IHC, IHC-P (10 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Suitable for use in ELISA and Western Blot. Western Blot: Stains a band of bone AP at ~90kD.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu ALPL

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Andere Bezeichnung: ALPL / Alkaline Phosphatase

Hintergrund: Name/Gene ID: ALPL

Synonyms: ALPL, Alkaline phosphomonoesterase, AP-TNAP, Alkaline phosphatase liver, APTNAP, Glycerophosphatase, Tissue-nonspecific ALP, TNAP, HOPS, Liver alkaline phosphatase, TNSALP

Gen-ID: 249

UniProt: P05186