HPGD Antikörper (AA 255-266)

Produktnummer ABIN462285

Der Ziege Polyklonal anti-HPGD Antikörper (ABIN462285) detektiert spezifisch HPGD in WB, ELISA, IHC und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human und Affe.

Kurzübersicht für HPGD Antikörper (AA 255-266) (ABIN462285)

Target: HPGD (Hydroxyprostaglandin Dehydrogenase 15-(NAD) (HPGD))

Reaktivität: Human, Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser HPGD Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-HPGD Antikörper

Bindungsspezifität: AA 255-266

Spezifität: Human HPGD / 15-PGDH.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset (100%) Monkey, Panda, Dog, Pig (83%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-DYDTTPFQAKTQ from the C-terminus of human HPGD / 15-PGDH (NP_000851.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset (100%), Monkey, Panda, Dog, Pig (83%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:128000), WB (0.1 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu HPGD

Target: HPGD (Hydroxyprostaglandin Dehydrogenase 15-(NAD) (HPGD))

Andere Bezeichnung: 15-PGDH / HPGD

Hintergrund: Name/Gene ID: HPGD

Synonyms: HPGD, 15-PGDH, PGDH, PGDH1, PHOAR1, Prostaglandin dehydrogenase 1, SDR36C1

Gen-ID: 3248

NCBI Accession: NP_000851

UniProt: P15428