CRABP1 Antikörper (AA 1-137)

Produktnummer ABIN462276

Produktdetails anti-CRABP1 Antikörper

Target: CRABP1 (Cellular Retinoic Acid Binding Protein 1 (CRABP1))

Bindungsspezifität: AA 1-137

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CRABP1 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human CRABP1 (1-137aa)

Aufreinigung: Protein G purified

Immunogen: Recombinant human CRABP1 (1-137aa) purified from E. coli

Type of Immunogen: Recombinant protein

Klon: AT1A1

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (1:500 - 1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Extracts of mouse eye (30 μg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CRABP1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Details zu CRABP1

Target: CRABP1 (Cellular Retinoic Acid Binding Protein 1 (CRABP1))

Andere Bezeichnung: CRABP1 / CRABP (CRABP1 Produkte)

Synonyme: CRABP antikoerper, CRABP-I antikoerper, CRABPI antikoerper, RBP5 antikoerper, CRABP1 antikoerper, cCRABP-I antikoerper, AI326249 antikoerper, Crabp-1 antikoerper, CrabpI antikoerper, Rbp-5 antikoerper, crabp1l antikoerper, crabpib antikoerper, crabp1 antikoerper, zgc:110497 antikoerper, cellular retinoic acid binding protein 1 antikoerper, cellular retinoic acid binding protein 1 S homeolog antikoerper, cellular retinoic acid binding protein I antikoerper, cellular retinoic acid binding protein 1b antikoerper, cellular retinoic acid binding protein 1a antikoerper, CRABP1 antikoerper, Crabp1 antikoerper, crabp1.S antikoerper, crabp1 antikoerper, crabp1b antikoerper, crabp1a antikoerper

Hintergrund: Name/Gene ID: CRABP1

Synonyms: CRABP1, CRABP-I, CRABPI, CRABP

Gen-ID: 1381

UniProt: P29762