CRABP1 Antikörper (AA 1-137)

Produktnummer ABIN462276

Dieser Anti-CRABP1 Antikörper ist ein Maus Monoklonal Antikörper zur Detektion von CRABP1 in WB, ELISA, IHC und IHC (p). Geeignet für Human und Maus.

Kurzübersicht für CRABP1 Antikörper (AA 1-137) (ABIN462276)

Target: CRABP1 (Cellular Retinoic Acid Binding Protein 1 (CRABP1))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CRABP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: AT1A1

Produktdetails anti-CRABP1 Antikörper

Bindungsspezifität: AA 1-137

Spezifität: Human CRABP1 (1-137aa)

Aufreinigung: Protein G purified

Immunogen: Recombinant human CRABP1 (1-137aa) purified from E. coli

Type of Immunogen: Recombinant protein

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (1:500 - 1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Extracts of mouse eye (30 μg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CRABP1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Details zu CRABP1

Target: CRABP1 (Cellular Retinoic Acid Binding Protein 1 (CRABP1))

Andere Bezeichnung: CRABP1 / CRABP

Hintergrund: Name/Gene ID: CRABP1

Synonyms: CRABP1, CRABP-I, CRABPI, CRABP

Gen-ID: 1381

UniProt: P29762