Glucose-6-Phosphate Dehydrogenase Antikörper (AA 35-506)

Produktnummer ABIN462273

Dieser Anti-Glucose-6-Phosphate Dehydrogenase Antikörper ist ein Maus Monoklonal Antikörper zur Detektion von Glucose-6-Phosphate Dehydrogenase in WB, IHC, ELISA und IHC (p). Geeignet für Human.

Kurzübersicht für Glucose-6-Phosphate Dehydrogenase Antikörper (AA 35-506) (ABIN462273)

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Glucose-6-Phosphate Dehydrogenase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: AT2F6

Produktdetails anti-Glucose-6-Phosphate Dehydrogenase Antikörper

Bindungsspezifität: AA 35-506

Spezifität: Human G6PD (35-506aa)

Aufreinigung: Protein G purified

Immunogen: Recombinant human G6PD (35-506aa) purified from E. coli

Type of Immunogen: Recombinant protein

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (1:1000 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Cell lysates of MCF7 (30 μg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human G6PD (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Details zu Glucose-6-Phosphate Dehydrogenase

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Andere Bezeichnung: G6PD

Hintergrund: Name/Gene ID: G6PD

Synonyms: G6PD, G6PD1

Gen-ID: 2539

UniProt: P11413

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones