RNH1 Antikörper (AA 7-461)

Produktnummer ABIN462271

Dieses Maus Monoklonal-Antikörper erkennt spezifisch RNH1 in WB, IHC, ELISA und IHC (p). Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für RNH1 Antikörper (AA 7-461) (ABIN462271)

Target: RNH1 (Ribonuclease/angiogenin Inhibitor 1 (RNH1))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser RNH1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: AT1H23

Produktdetails anti-RNH1 Antikörper

Bindungsspezifität: AA 7-461

Marke: IHC-plus™

Spezifität: Ribonuclease inhibitor 1(RNH1) (7-461aa)

Aufreinigung: Protein G purified

Immunogen: Recombinant Ribonuclease inhibitor 1(RNH1) (7-461aa) purified from E. coli

Type of Immunogen: Recombinant protein

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (1:1000 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Extracts of mouse liver (20 μg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human RNH1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Details zu RNH1

Target: RNH1 (Ribonuclease/angiogenin Inhibitor 1 (RNH1))

Andere Bezeichnung: RNH1

Hintergrund: Name/Gene ID: RNH1

Synonyms: RNH1, Placental RNase inhibitor, PRI, RAI, Ribonuclease inhibitor, RNH

Gen-ID: 6050

UniProt: P13489