Tyrosine Hydroxylase Antikörper

Produktnummer ABIN462131

Produktdetails anti-Tyrosine Hydroxylase Antikörper

Target: Tyrosine Hydroxylase (TH)

Reaktivität: Human, Ratte, Maus, Cat, Frettchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Tyrosine Hydroxylase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Marke: IHC-plus™

Spezifität: Recognizes tyrosine hydroxylase. Species cross-reactivity: Mouse, human, ferret, feline and Aplysia (marine mollusc). Because Tyrosine Hydroxylase is highly conserved, this antibody is expected to cross-react with most mammalian species and many non-mammalian species.

Aufreinigung: Immunoaffinity purified

Immunogen: Denatured tyrosine hydroxylase from rat pheochromocytoma (denatured by sodium dodecyl sulfate).

Type of Immunogen: Purified protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IF (1:100 - 1:1000), IHC, IHC-Fr, IHC-P (1:100), IP, WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. Western Blot: 1:1000 on 10 μg of PC12 lysates. Immunofluorescence: 1:100-1:1000. Fixed with 4 % PFA overnight at 4C.

Kommentare:

Target Species of Antibody: Rat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Tyrosine Hydroxylase

Target: Tyrosine Hydroxylase (TH)

Andere Bezeichnung: TH / Tyrosine Hydroxylase (TH Produkte)

Synonyme: DYT14 antikoerper, DYT5b antikoerper, TYH antikoerper, The antikoerper, CG10118 antikoerper, DH65B antikoerper, DTH antikoerper, Dmel\\CG10118 antikoerper, Pale antikoerper, Ple antikoerper, TH antikoerper, Th antikoerper, VII antikoerper, dTH1 antikoerper, dTH65B antikoerper, pale/ple antikoerper, th antikoerper, dyt14 antikoerper, dyt5b antikoerper, tyh antikoerper, tyrosine hydroxylase antikoerper, pale antikoerper, tyrosine 3-monooxygenase antikoerper, tyrosine hydroxylase S homeolog antikoerper, TH antikoerper, Th antikoerper, ple antikoerper, th antikoerper, th.S antikoerper, LOC100008895 antikoerper

Hintergrund: Name/Gene ID: TH
Family: Monooxygenase

Synonyms: TH, DYT14, Dystonia 14, TYH, Tyrosine 3-hydroxylase, Tyrosine hydroxylase, DYT5b, Tyrosine 3-monooxygenase

Gen-ID: 7054

UniProt: P07101

Pathways: Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour