ICAM1 Antikörper

Produktnummer ABIN462119

Der Maus-Monoklonal Anti-ICAM1-Antikörper wurde für FACS, IHC, IP, IHC (p) und IHC (fro) validiert. Er ist geeignet zum Nachweis von ICAM1 in Proben aus Human und Schwein. }

Kurzübersicht für ICAM1 Antikörper (ABIN462119)

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reaktivität: Human, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ICAM1 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: 15-2

Produktdetails anti-ICAM1 Antikörper

Spezifität: Recognizes the human CD54 cell surface antigen, a 90kD glycoprotein also known as ICAM-1. CD54 is expressed by many cells following activation by inflammatory mediators. Is reported to block CD54 function.

Aufreinigung: Protein G purified

Immunogen: Human monocytes.

Type of Immunogen: Cells

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: Flo (1:50 - 1:100), IHC, IHC-Fr, IHC-P (10 μg/mL), IP

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu ICAM1

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Andere Bezeichnung: ICAM1 / CD54

Hintergrund: Name/Gene ID: ICAM1
Family: ICAM

Synonyms: ICAM1, BB2, CD54 antigen, CD54, ICAM-1, p3.58

Gen-ID: 3383

UniProt: P05362

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling