RAB3A Antikörper (AA 202-217)

Produktnummer ABIN462094

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch RAB3A in WB, IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human und Affe.

Kurzübersicht für RAB3A Antikörper (AA 202-217) (ABIN462094)

Target: RAB3A (RAB3A, Member RAS Oncogene Family (RAB3A))

Reaktivität: Human, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RAB3A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-RAB3A Antikörper

Bindungsspezifität: AA 202-217

Marke: IHC-plus™

Spezifität: Detects an ~25 kD protein, corresponding to the apparent molecular mass of Rab3A on SDSPAGE immunoblots, in samples from mouse and rat origins. The antibody specificity is confirmed in peptide inhibition immunoblotting studies.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%) Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Platypus, Xenopus (88%).

Aufreinigung: Immunoaffinity purified

Immunogen: A 16 residue synthetic peptide (C)KQGPQLSDQQVPPHQD based on the human Rab3A (residues 202-217) with the cysteine (C) residue added and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%), Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Platypus, Xenopus (88%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (10 μg/mL), WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Borate buffered saline,  pH 8.2 (25  mM sodium borate, 100  mM boric acid, 75  mM NaCl, 5  mM EDTA (disodium salt)

Handhabung: avoid freeze thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Details zu RAB3A

Target: RAB3A (RAB3A, Member RAS Oncogene Family (RAB3A))

Andere Bezeichnung: RAB3A

Hintergrund: Name/Gene ID: RAB3A
Subfamily: RAS oncogene
Family: Ras GTPase superfamily IPR001806

Synonyms: RAB3A, RAS-associated protein RAB3A, Ras-related protein Rab-3A

Gen-ID: 5864

UniProt: P20336

Pathways: Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport