CXCR4 Antikörper (AA 14-40)

Produktnummer ABIN461996

Der Ziege Polyklonal Anti-CXCR4-Antikörper wurde für WB, ELISA, IHC und IHC (p) validiert. Er ist geeignet, CXCR4 in Proben von Human und Maus zu detektieren.

Kurzübersicht für CXCR4 Antikörper (AA 14-40) (ABIN461996)

Target: CXCR4 (Chemokine (C-X-C Motif) Receptor 4 (CXCR4))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CXCR4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-CXCR4 Antikörper

Bindungsspezifität: AA 14-40

Marke: IHC-plus™

Spezifität: This antibody binds to CXCR4 receptor on mouse spleen leukocytes as determined by immunocytochemistry.

Homologie: Percent identity by BLAST analysis: Mouse (100%) Rat (93%) Baboon, Monkey, Marmoset, Elephant, Rabbit (81%).

Aufreinigung: Immunoaffinity purified

Immunogen: YSEEVGSGDYDSNKEPCFRDENVHFNR corresponding to the N-terminal extracellular domain of mouse CXCR4 receptor. Percent identity by BLAST analysis: Mouse (100%), Rat (93%), Baboon, Monkey, Marmoset, Elephant, Rabbit (81%).

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:25000), IHC, IHC-P (5 μg/mL), WB (1:500)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Phosphate buffered saline, 1 mg/mL BSA, 0.1 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu CXCR4

Target: CXCR4 (Chemokine (C-X-C Motif) Receptor 4 (CXCR4))

Andere Bezeichnung: CXCR4

Hintergrund: Name/Gene ID: CXCR4
Subfamily: Chemokine
Family: GPCR

Synonyms: CXCR4, CD184, CD184 antigen, Cmkar4, Cxc chemokine receptor 4, CXCR-4, CXC-R4, D2S201E, FB22, Fusin, HM89, LCR1, NPY3R, NPYRL, NPYY3R, Npyy3, HSY3RR, SDF-1 receptor, WHIM, C-x-c chemokine receptor 4, LESTR, Neuropeptide Y receptor Y3, NPYR

Gen-ID: 7852

UniProt: P61073

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events