Pyrophosphatase (Inorganic) 1 (PPA1) Antikörper

Produktnummer ABIN459215

Produktdetails

Target: Pyrophosphatase (Inorganic) 1 (PPA1)

Reaktivität: Saccharomyces cerevisiae

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Spezifität: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Produktmerkmale: IgG fraction of polyclonal rabbit antiserum to inorganic pyrophosphatase from yeast

Aufreinigung: The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogen: Inorganic pyrophosphatase is isolated and purified from yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: IgG protein concentration 10 mg/ml. No foreign proteins added.

Buffer: Purified hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilised IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Referenzen

Patir-Nebioglu, Andrés, Krebs, Fink, Drzewicka, Stankovic-Valentin, Segami, Schuck, Büttner, Hell, Maeshima, Melchior, Schumacher: "Pyrophosphate modulates plant stress responses via SUMOylation." in: eLife, Vol. 8, (2019) (PubMed).

Antigendetails

Target: Pyrophosphatase (Inorganic) 1 (PPA1)

Andere Bezeichnung: Inorganic Pyrophosphatase (PPA1 Produkte)

Synonyme: ppa antikoerper, SCE9.16 antikoerper, PSPTO0722 antikoerper, AO090005001437 antikoerper, IOPPP antikoerper, PP antikoerper, PP1 antikoerper, SID6-8061 antikoerper, 2010317E03Rik antikoerper, Pyp antikoerper, Pp antikoerper, inorganic pyrophosphatase antikoerper, Inorganic pyrophosphatase antikoerper, pyrophosphatase (inorganic) 1 L homeolog antikoerper, pyrophosphatase (inorganic) 1 antikoerper, ppa antikoerper, SCO3409 antikoerper, ppa-1 antikoerper, APE_1692.1 antikoerper, AOR_1_2492174 antikoerper, Celal_2154 antikoerper, Celly_1958 antikoerper, Dester_1338 antikoerper, Marky_0054 antikoerper, Halhy_5834 antikoerper, ppa1.L antikoerper, PPA1 antikoerper, Ppa1 antikoerper

Substanzklasse: Viral Protein

Hintergrund: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.