Pyrophosphatase (Inorganic) 1 (PPA1) Antikörper

Produktnummer ABIN459215

Es gibt 1 Publikation für dieses Produkt. Der Kaninchen Polyklonal anti-Pyrophosphatase (Inorganic) 1 Antikörper wird verwendet zum Nachweis von Pyrophosphatase (Inorganic) 1 in Proben von Saccharomyces cerevisiae. Er wurde validiert für WB, ELISA, IF und DB.

Kurzübersicht für Pyrophosphatase (Inorganic) 1 (PPA1) Antikörper (ABIN459215)

Target: Pyrophosphatase (Inorganic) 1 (PPA1)

Reaktivität: Saccharomyces cerevisiae

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Dot Blot (DB)

Produktdetails

Verwendungszweck: Rabbit anti Saccharomyces cerevisiae Inorganic pyrophosphatase

Spezifität: IgG fraction of polyclonal rabbit antiserum to inorganic pyrophosphatase from yeast.

Kreuzreaktivität (Details): Cross-reactivities against enzymes of other sources may occur but have not been determined.

Produktmerkmale: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer's own choice.

Aufreinigung: Purified IgG fraction from antiserum

Immunogen: Inorganic pyrophosphatase is isolated and purified from yeast. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Indirect immunofluoresence,ELISA,Dot blot,Immunoblotting.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is reconstituted by adding 1.0 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: Purified hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The lyophilised IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Referenzen

Patir-Nebioglu, Andrés, Krebs, Fink, Drzewicka, Stankovic-Valentin, Segami, Schuck, Büttner, Hell, Maeshima, Melchior, Schumacher: "Pyrophosphate modulates plant stress responses via SUMOylation." in: eLife, Vol. 8, (2019) (PubMed).

Antigendetails

Target: Pyrophosphatase (Inorganic) 1 (PPA1)

Andere Bezeichnung: Inorganic Pyrophosphatase

UniProt: P00817