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Nuclease P1 Antikörper (Biotin)

Reaktivität: Penicillium sp. ELISA, WB, IF Wirt: Kaninchen Polyclonal Biotin
Produktnummer ABIN459081
  • Target Alle Nuclease P1 Produkte
    Nuclease P1
    Reaktivität
    • 7
    • 6
    • 4
    Penicillium sp.
    Wirt
    • 15
    Kaninchen
    Klonalität
    • 15
    Polyklonal
    Konjugat
    • 6
    • 5
    • 2
    • 2
    Dieser Nuclease P1 Antikörper ist konjugiert mit Biotin
    Applikation
    • 12
    • 7
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    ELISA, Western Blotting (WB), Immunofluorescence (IF)
    Spezifität
    Cross-reactivities against enzymes of other sources may occur but have not been determined.
    Produktmerkmale
    Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to nuclease P1 from Penicillium citrinum
    Aufreinigung
    The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
    Immunogen
    Nuclease P1 isolated and purified from Penicillium citrinum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
    Isotyp
    IgG
  • Applikationshinweise
    This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immuno-fluorescence or histochemical techniques).
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Konzentration
    IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 6.5. No foreign proteins added.
    Buffer
    Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
    Konservierungsmittel
    Without preservative
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to us e, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day . If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
  • Target
    Nuclease P1
    Abstract
    Nuclease P1 Produkte
    Hintergrund
    The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
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