ALT Antikörper (Biotin)

Produktnummer ABIN459041

Produktdetails anti-ALT Antikörper (Biotin)

Target: ALT (Alanine Aminotransferase (ALT))

Reaktivität: Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ALT Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Spezifität: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Produktmerkmale: Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to glutamic-pyruvic transaminase from porcine heart

Aufreinigung: The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogen: Glutamic-pyruvic transaminase isolated and purified from porcine heart. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 4.2. No foreign proteins added.

Buffer: Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Details zu ALT

Target: ALT (Alanine Aminotransferase (ALT))

Andere Bezeichnung: Glutamic-pyruvic Transaminase (ALT Produkte)

Synonyme: AAT1 antikoerper, ALT1 antikoerper, GPT1 antikoerper, GPT antikoerper, Gpt1 antikoerper, An11g02620 antikoerper, AO090003000164 antikoerper, 233.t00009 antikoerper, 24.t00016 antikoerper, 1300007J06Rik antikoerper, 2310022B03Rik antikoerper, ALT antikoerper, Gpt-1 antikoerper, AU021132 antikoerper, Dpagt2 antikoerper, Gnpta antikoerper, gpt antikoerper, ALANINE AMINOTRANSFERAS antikoerper, AtAlaAT1 antikoerper, AtAlaATc antikoerper, T13M22.3 antikoerper, T13M22_3 antikoerper, alanine aminotransferas antikoerper, glutamic--pyruvic transaminase antikoerper, glutamic-pyruvate transaminase (alanine aminotransferase) antikoerper, alanine aminotransferase antikoerper, alanine aminotransferase 1 antikoerper, glutamic pyruvic transaminase, soluble antikoerper, dolichyl-phosphate (UDP-N-acetylglucosamine) acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) antikoerper, alanine aminotransferase 2-like antikoerper, Alanine aminotransferase 1, mitochondrial antikoerper, GPT antikoerper, Gpt antikoerper, Tb927.1.3950 antikoerper, ANI_1_368094 antikoerper, AOR_1_284154 antikoerper, ALAT_1 antikoerper, ALAAT1 antikoerper, EHI_159710 antikoerper, EHI_096750 antikoerper, LOC5572541 antikoerper, ALAT antikoerper, AAT2 antikoerper, Dpagt1 antikoerper, LOC100537633 antikoerper, AlaAT1 antikoerper

Hintergrund: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.