Clostripain Antikörper (Biotin)

Produktnummer ABIN459023

Der Kaninchen Polyklonal anti-Clostripain Antikörper wird verwendet zum Nachweis von Clostripain in Proben von Clostridium histolyticum. Er wurde validiert für WB, ELISA, DB, IHC (p) und ICC.

Kurzübersicht für Clostripain Antikörper (Biotin) (ABIN459023)

Target: Clostripain (closI)

Reaktivität: Clostridium histolyticum

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Clostripain Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Dot Blot (DB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Produktdetails anti-Clostripain Antikörper (Biotin)

Verwendungszweck: Rabbit anti Clostridium histolyticum Clostripain, conjugated with Biotin

Spezifität: Biotin-conjugated IgG fraction of polyclonal Rabbit antiSerum to clostripain from Clostridium histolyticum.

Kreuzreaktivität (Details): Cross-reactivities against enzymes of other sources may occur but have not been determined.

Produktmerkmale: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).

Aufreinigung: Purified IgG fraction from antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogen: Clostipain is isolated and purified from Clostridium histolyticum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is reconstituted by adding 1.0 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Details zu Clostripain

Target: Clostripain (closI)

Andere Bezeichnung: Clostripain

UniProt: P09870