Peroxidase Antikörper

Produktnummer ABIN458943

Dieses Anti-Peroxidase-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Peroxidase in WB, ELISA und IF. Geeignet für Meerrettich.

Kurzübersicht für Peroxidase Antikörper (ABIN458943)

Target: Peroxidase

Reaktivität: Meerrettich

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Peroxidase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Produktdetails anti-Peroxidase Antikörper

Spezifität: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Produktmerkmale: Affinity purified antibodies of polyclonal rabbit antiserum to peroxidase from horseradish

Aufreinigung: Specific polyclonal antibody fractions were prepared by solid phase affinity chromatography.

Immunogen: Peroxidase isolated and purified from horseradish. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Anwendungsinformationen

Applikationshinweise: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: PAb concentration 1 mg/0.5ml. No foreign proteins added.

Buffer: Purified hyperimmune rabbit IgG antibodies lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2), stabilized with dextran.

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilised PAb fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 0.5 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Details zu Peroxidase

Target: Peroxidase

Hintergrund: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.