Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Glycerol Dehydrogenase in IF, WB und ELISA. Er zeigt eine Reaktivität gegenüber Klebsiella aerogenes.
Produktnummer ABIN458914
Kurzübersicht für Glycerol Dehydrogenase Antikörper (ABIN458914)
Target
Glycerol Dehydrogenase
Reaktivität
Klebsiella aerogenes
Wirt
Kaninchen
Klonalität
Polyklonal
Konjugat
Dieser Glycerol Dehydrogenase Antikörper ist unkonjugiert
Applikation
Immunofluorescence (IF), Western Blotting (WB), ELISA
Spezifität
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Produktmerkmale
Affinity purified antibodies of polyclonal rabbit antiserum to glycerol dehydrogenase from Enterobacter aerogenes
Aufreinigung
Specific polyclonal antibody fractions were prepared by solid phase affinity chromatography.
Immunogen
Glycerol dehydrogenase isolated and purified from Enterobacter aerogenes. Freund’s complete adjuvant is used in the first step of the immunization procedure.
This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Konzentration
PAb concentration 1 mg/0.5ml. No foreign proteins added.
Buffer
Purified hyperimmune rabbit IgG antibodies lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2), stabilized with dextran.
Konservierungsmittel
Without preservative
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
The lyophilised PAb fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 0.5 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Target
Glycerol Dehydrogenase
Hintergrund
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.