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FITC Antikörper

FITC ELISA, WB Wirt: Maus Monoclonal NI 239 unconjugated
Produktnummer ABIN458849
  • Target Alle FITC Produkte
    FITC (Fluorescein Isothiocyanate (FITC))
    Reaktivität
    Bitte anfragen
    Wirt
    • 47
    • 17
    • 16
    • 8
    • 6
    Maus
    Klonalität
    • 54
    • 39
    Monoklonal
    Konjugat
    • 31
    • 14
    • 13
    • 12
    • 7
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser FITC Antikörper ist unkonjugiert
    Applikation
    • 53
    • 40
    • 32
    • 18
    • 16
    • 15
    • 14
    • 6
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Western Blotting (WB)
    Spezifität
    This antiserum has not been tested for cross-reactivity with other fluorescent compounds.
    Produktmerkmale
    Monoclonal mouse antibody to fluorescein isothiocyanate isomer 1
    Immunogen
    Highly purified fluorescein isothiocyanate isomer 1.
    Klon
    NI 239
    Isotyp
    IgG1 kappa
  • Applikationshinweise
    To enhance the specific signal obtained with a monoclonal antibody or a polyclonal second antibody conjugated to FITC. The phenomenon of a weak reaction of a monoclonal antibody is e.g. well known in different analysis of vital peripheral blood mononuclear cells in suspensions by the expression of surface markers. A similar situation exists in solid phase assay systems (ELISA, blotting, DIBA) when used for the identification and /or quantitative determination of minute amounts of soluble specific antigens or antibodies. The sensitivity of the FITC hapten-anti-hapten system makes it a valuable alternative to the biotin-avidin system. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Konzentration
    IgG concentration is 1.0 mg/ml. No foreign proteins added.
    Buffer
    Delipidated, heat inactivated, lyophilized, stable whole ascites.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below - 20 °C. Reconstitute the lyophilized ascites by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4 °C, not refrozen, and preferably used the same day.
  • Target
    FITC (Fluorescein Isothiocyanate (FITC))
    Andere Bezeichnung
    Fluorescein Isothiocyanate Isomer 1 (FITC Produkte)
    Substanzklasse
    Chemical
    Hintergrund
    The reactivity of the antiserum is directed to the FITC molecule as tested in direct binding enzyme immunoassay, ELISA and immuno-blotting.
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