Tetramethylrhodamine Isothiocyanate Isomer R Antikörper

Produktnummer ABIN458843

Produktdetails

Target: Tetramethylrhodamine Isothiocyanate Isomer R

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: ELISA, Western Blotting (WB)

Spezifität: This antiserum has not been tested for cross-reactivity with other fluorescent compounds.

Produktmerkmale: Purified monoclonal mouse antibody to tetramethylrhodamine isothiocyanate isomer R

Aufreinigung: Purified

Immunogen: Highly purified fluorescein isothiocyanate isomer 1.

Klon: NI 308

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: To enhance the specific signal obtained with a monoclonal antibody or a polyclonal second antibody conjugated to TRITC. The phenomenon of a weak reaction of a monoclonal antibody is e.g. well known in different analysis of vital peripheral blood mononuclear cells in suspensions by the expression of surface markers. A similar situation exists in solid phase assay systems (ELISA, blotting, DIBA) when used for the identification and /or quantitative determination of minute amounts of soluble specific antigens or antibodies. The sensitivity of the TRITC hapten-anti-hapten system makes it a valuable alternative to the biotin-avidin system. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:40, in ELISA from 1:100 upwards, in Western blotting from 1:200 upwards. These data should be interpreted as general recommendations only.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: IgG concentration is 0.4 mg/ml. No foreign proteins added.

Buffer: Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2).

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below - 20 °C. Reconstitute the lyophilized ascites by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4 °C, not refrozen, and preferably used the same day.

Antigendetails

Target: Tetramethylrhodamine Isothiocyanate Isomer R

Substanzklasse: Chemical

Hintergrund: The reactivity of the antiserum is directed to the TRITC molecule as tested in direct binding enzyme immunoassay, ELISA and immunoblotting.