HRP Antikörper

Produktnummer ABIN458842

Produktdetails anti-HRP Antikörper

Target: HRP (Horseradish Peroxidase (HRP))

Reaktivität: Meerrettich

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HRP Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB)

Spezifität: This antiserum has not been tested for cross-reactivity.

Produktmerkmale: Purified monoclonal mouse antibody to horseradish peroxidase

Aufreinigung: Purified

Immunogen: Highly purified horseradish peroxidase

Klon: NI 91

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: TAs a control in the tests where mouse IgG1 (with irrelevant specificity) should be used to show the presence of that given mouse isotype. In indirect tests as reagent to horseradish peroxidase or to potentiatethe visualisation of a peroxidase labelled conjugate in the appropriate material. As secondary reagent a labelled anti mouse IgG will serve for the detection. This system can be further be potentiated by the use of anti-FITC or anti-TRITC conjugates. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: IgG concentration is 0.4 mg/ml. No foreign proteins added.

Buffer: Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2).

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below - 20 °C. Reconstitute the lyophilized ascites by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4 °C, not refrozen, and preferably used the same day.

Details zu HRP

Target: HRP (Horseradish Peroxidase (HRP))

Andere Bezeichnung: Horseradish Peroxidase (HRP Produkte)

Hintergrund: The reactivity of the antiserum is directed to horseradish as tested in direct binding enzyme immunoassay, ELISA and immunoblotting.