BETAHYDROXYSTEROID DEHYDROGENASE Antikörper (Biotin)

Details zu Produkt Nr. ABIN458821
Target
Reaktivität
Pseudomonas testosteroni
1
Wirt
Kaninchen
1
Klonalität
Polyklonal
Konjugat
Biotin
Applikation
Immunofluorescence (IF), ELISA, Western Blotting (WB)
1
1
1
Optionen
Immunogen beta -Hydroxysteroid dehydrogenase isolated and purified from Pseudomonas testosteroni cells. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Isotyp IgG
Spezifität Cross-reactivities against enzymes of other sources may occur but have not been determined.
Produktmerkmale Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to β-hydroxysteroid dehydrogenase from Pseudomonas testosteroni cells
Reinigung The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
Target
Andere Bezeichnung beta-hydroxysteroid dehydrogenase
Hintergrund The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
Applikations-hinweise This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Konzentration IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 5.2. No foreign proteins added.
Buffer Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
Konservierungs-mittel Without preservative
Lagerung 4 °C/-20 °C
Informationen zur Lagerung The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slow ly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.