Erythrocytes Antikörper

Produktnummer ABIN458786

Produktdetails

Target: Erythrocytes

Reaktivität: Schaf

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Screening Assay (ScA)

Spezifität: Homologous proteins of different species frequently share antigenic determinants. has not been tested.

Produktmerkmale: IgG fraction of polyclonal rabbit antiserum to sheep erythrocytes

Aufreinigung: Adsorption: No adsorption required. Cross-reactivity Homologous proteins of different species frequently share antigenic determinants. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.

Immunogen: Washed whole erythrocytes were prepared from fresh blood and used for intravenous immunization. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: To sensitize cells in haemolytic complement assays and for screening of T and T receptor-bearing lymphocytes.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: IgG protein concentration 10 mg/ml. No foreign proteins added. Antibody titre: Haemolytic titre, at which 50% lysis occurs following mixing of equal volumes of antiserum, guinea pig complement (at a 1:60 dilution) and 5x10 8 RBC/ml, not less than 1:3,000

Buffer: Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.

Antigendetails

Target: Erythrocytes