alpha Fetoprotein Antikörper (FITC)

Produktnummer ABIN458738

Der Schaf Polyklonal anti-alpha Fetoprotein Antikörper wird verwendet zum Nachweis von alpha Fetoprotein in Proben von Ratte. Er wurde validiert für ELISA, ICC, IHC (fro) und IFA.

Kurzübersicht für alpha Fetoprotein Antikörper (FITC) (ABIN458738)

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reaktivität: Ratte

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser alpha Fetoprotein Antikörper ist konjugiert mit FITC

Applikation: ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Indirect Immunofluorescence Assay (IFA)

Produktdetails anti-alpha Fetoprotein Antikörper (FITC)

Verwendungszweck: Sheep anti Rat alpha foetoprotein, conjugated with FITC

Spezifität: Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Sheep antiSerum to Rat alpha foetoprotein.

Kreuzreaktivität (Details): Inter-species cross-reactivity is a normal feature of antibodies to mammalian proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, however in double radial immunodiffusion (Ouchterlony) a precipitation reaction has been observed with Mouse amniotic fluid.

Produktmerkmale: The reactivity of the antiserum is restricted to alpha-foetoprotein. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against amniotic fluid, rat hepatoma sera and purified alpha-foetoprotein an single characteristic precipitin line is obtained. No reaction is obtained with rat sera of different strains. To identify and measure alpha foetoprotein at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates and to identify and measure alpha-foetoprotein in rat serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Aufreinigung: Purified IgG fraction from antiserum

Immunogen: Purified rat alpha-foetoprotein is isolated from pooled amniotic fluid and hepatoma serum using a proprietary multiple step method. Several foetoproteins have been demonstrated in rat foetal serum and amniotic fluid. One of the best studied is the alpha-foetoprotein. It can be easily identified immunochemically because of its distinct antigenicity. In electrophoresis its relative mobility is between the albumin and the alpha-1 globulin fractions. Its molecular weight is 65,000, the carbohydrate content is relatively low. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: FITC-coupled purified hyperimmune sheep IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the activity of the conjugate.

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Details zu alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Andere Bezeichnung: alpha Foetoprotein

UniProt: P02773

Pathways: C21-Steroid Hormone Metabolic Process