Transferrin Antikörper (HRP)
Kurzübersicht für Transferrin Antikörper (HRP) (ABIN458566)
Target
Alle Transferrin (TF) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Verwendungszweck
- Rabbit anti Swine transferrin, conjugated with Horseradish peroxidase
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Spezifität
- Horseradish peroxidase-conjugated IgG fraction of polyclonal Rabbit antiSerum to Swine transferrin.
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Kreuzreaktivität (Details)
- Inter-species cross-reactivity is a normal feature of antibodies to Serum proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
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Produktmerkmale
- The reactivity of the antiserum is restricted to transferrin as tested in immunoelectrophoresis and radial immunodiffusion. A characteristic single precipitin line is obtained with normal serum which shows a reaction of identity with the precipitin line with purified transferrin. In enzyme-immunocytochemical and immunohistochemical techniques for the detection of transferrin at the cellular and subcellular level in appropriately treated cell and tissue substrates, as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA, Western blotting). This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
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Aufreinigung
- Purified IgG fraction from antiserum
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Immunogen
- Purified transferrin isolated from pooled swine serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
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Isotyp
- IgG
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Applikationshinweise
- ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.
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Buffer
- Horseradish peroxidase-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added
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Konservierungsmittel
- Without preservative
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- The lyophilized immunoconjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.
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- Transferrin (TF)
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Andere Bezeichnung
- Transferrin
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Pathways
- Transition Metal Ion Homeostasis
Target
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