Transferrin Antikörper (FITC)

Produktnummer ABIN458564

Der Kaninchen Polyklonal anti-Transferrin Antikörper wird verwendet zum Nachweis von Transferrin in Proben von Schwein. Er wurde validiert für ELISA, ICC, IHC (fro) und IFA.

Kurzübersicht für Transferrin Antikörper (FITC) (ABIN458564)

Target: Transferrin (TF)

Reaktivität: Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Transferrin Antikörper ist konjugiert mit FITC

Applikation: ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Indirect Immunofluorescence Assay (IFA)

Produktdetails anti-Transferrin Antikörper (FITC)

Verwendungszweck: Rabbit anti Swine transferrin, conjugated with FITC

Spezifität: Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Rabbit antiSerum to Swine transferrin.

Kreuzreaktivität (Details): Inter-species cross-reactivity is a normal feature of antibodies to Serum proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

Produktmerkmale: The reactivity of the antiserum is restricted to transferrin as tested in immunoelectrophoresis and radial immunodiffusion. A characteristic single precipitin line is obtained with normal serum which shows a reaction of identity with the precipitin line with purified transferrin. In immunocytochemical and immunohistochemical assays to identify and measure transferrin at the cellular and subcellular level by fluorescent staining of appropriately treated cell and tissue substrates. In non-isotopic assay methodology (e.g. ELISA and Western blotting) to identify and measure transferrin in swine serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Aufreinigung: Purified IgG fraction from antiserum

Immunogen: Purified transferrin isolated from pooled swine serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.

Buffer: FITC-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The lyophilized immunoconjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

Details zu Transferrin

Target: Transferrin (TF)

Andere Bezeichnung: Transferrin

Pathways: Transition Metal Ion Homeostasis