Fibrinogen Antikörper

Produktnummer ABIN458335

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch Fibrinogen in IEP, ID und RID. Er zeigt eine Reaktivität gegenüber Maus und wurde in 5+ Publikationen erwähnt.

Kurzübersicht für Fibrinogen Antikörper (ABIN458335)

Target: Fibrinogen

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Fibrinogen Antikörper ist unkonjugiert

Applikation: Immunoelectrophoresis (IEP), Immunodiffusion (ID), Radial Immunodiffusion (RID)

Produktdetails anti-Fibrinogen Antikörper

Spezifität: The antiserum does not cross-react with any other component of mouse plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. of this antiserum has not been tested in detail.

Produktmerkmale: Precipitating polyclonal goat antiserum to mouse fibrinogen

Aufreinigung: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogen: Fibrinogen (clotting factor I) is a heat labile beta glycoprotein present in plasma. It is the precursor of fibrin, which is the key protein constituting the network of the blood clot. Thrombin converts fibrinogen to fibrin by limited proteolysis. Fibrin monomers polymerize to fibrin which is stabilized by cross-linking. Fibrinogen is isolated from fresh plasma after removing prothrombin. Freund’s complete adjuvant is used in the first step of the immuni-zation procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: In precipitating techniques as immunoelectrophoresis and single or double radial immunodiffusion (Mancini, Ouchterlony) to identify the presence of fibrinogen in mouse plasma or other body fluids or to determine its concentration.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added. Antibody titre: Precipitin titre 1:32 when tested against pooled normal mouse plasma in agar-block immunodiffusion titrat

Buffer: Delipidated, heat inactivated, lyophilized, stable whole antiserum

Konservierungsmittel: Without preservative

Lagerung: 4 °C

Referenzen für anti-Fibrinogen Antikörper (ABIN458335)

Miles, Baik, Lighvani, Khaldoyanidi, Varki, Bai, Mueller, Parmer: "Deficiency of plasminogen receptor, Plg-RKT , causes defects in plasminogen binding and inflammatory macrophage recruitment in vivo." in: Journal of thrombosis and haemostasis : JTH, Vol. 15, Issue 1, pp. 155-162, (2018) (PubMed).

Zang, Gustafsson, Jamalpour, Hong, Genové, Welsh: "Vascular dysfunction and increased metastasis of B16F10 melanomas in Shb deficient mice as compared with their wild type counterparts." in: BMC cancer, Vol. 15, pp. 234, (2016) (PubMed).

Díaz-Trelles, Scimia, Bushway, Tran, Monosov, Monosov, Peterson, Rentschler, Cabrales, Ruiz-Lozano, Mercola: "Notch-independent RBPJ controls angiogenesis in the adult heart." in: Nature communications, Vol. 7, pp. 12088, (2016) (PubMed).

Tugues, Roche, Noguer, Orlova, Bhoi, Padhan, Akerud, Honjo, Selvaraju, Mazzone, Tolmachev, Claesson-Welsh: "Histidine-rich glycoprotein uptake and turnover is mediated by mononuclear phagocytes." in: PLoS ONE, Vol. 9, Issue 9, pp. e107483, (2014) (PubMed).

Lin, Kwong, Warren, Wood, Bhatia: "Nanoparticles that sense thrombin activity as synthetic urinary biomarkers of thrombosis." in: ACS nano, Vol. 7, Issue 10, pp. 9001-9, (2014) (PubMed).

Details zu Fibrinogen

Target: Fibrinogen

Hintergrund: The reactivity of the antiserum is restricted to mouse fibrinogen molecule. In immuno-electrophoresis and radial immunodiffusion, using various antiserum concentrations against normal mouse plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified fibrinogen. No reaction is obtained with any other plasma protein components or serum. However, the antiserum may also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products