IgA Secretory Component (Free & Bound) Antikörper

Produktnummer ABIN458282

Es gibt 1 Publikation für dieses Produkt. Der Ziege Polyklonal anti-IgA Secretory Component Antikörper wird verwendet zum Nachweis von IgA Secretory Component in Proben von Affe. Er wurde validiert für ELISA, IEP und RID.

Kurzübersicht für IgA Secretory Component (Free & Bound) Antikörper (ABIN458282)

Target: IgA Secretory Component

Reaktivität: Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: ELISA, Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID)

Produktdetails

Bindungsspezifität: Free & Bound

Verwendungszweck: Goat anti Monkey secretory component (free and bound)

Spezifität: Precipitating polyclonal goat antiserum to monkey secretory component.

Kreuzreaktivität (Details): Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Precipitation reactions have been observed with free and bound secretory component in serum of other old-world Monkeys, including Cercopithecus, Cynomolgus and Baboon. The antiserum may also react with other species as has been observed for Chimpanzee.

Produktmerkmale: Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65,000). The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells. SC also has an affinity for polymeric IgM.

Aufreinigung: Delipidated, heat inactivated, lyophilized, stable whole antiserum

Immunogen: Purified free secretory component isolated from pooled rhesus monkey milk is used for immunization. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Precipitation assays. In immunoelectrophoresis use 2 μL serum or equivalent against 120 μL antiserum. In double radial immunodiffusion use a rosette arrangement with 10 μL antiserum in 3 mm diameter centre well and 2 μL serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. Tested in immunoelectrophoresis, double radial immunodiffusion and ELISA against a panel of appropriate secretions and purified Ig isotypes. The antiserum reacts with both bound secretory component (secretory IgA) and with the free SC present in monkey secretions. In immunoelectrophoresis against monkey milk, using a high electroendosmosis agar plate, free SC is precipitated in the alph-2 region. The antiserum does not react with other molecular forms of IgA, or with any other secretory or plasma protein. In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion to identify the presence secretory component in monkey serum or other body fluids and to determine its concentration.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.

Buffer: Delipidated, heat inactivated, lyophilized, stable whole antiserum No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

Referenzen

Thomas, Tuero, Demberg, Vargas-Inchaustegui, Musich, Xiao, Venzon, LaBranche, Montefiori, DiPasquale, Reed, DeVico, Fouts, Lewis, Gallo, Robert-Guroff: "HIV-1 CD4-induced (CD4i) gp120 epitope vaccines promote B and T-cell responses that contribute to reduced viral loads in rhesus macaques." in: Virology, Vol. 471-473, pp. 81-92, (2015) (PubMed).

Antigendetails

Target: IgA Secretory Component

Andere Bezeichnung: Secretory Immunoglobulin A (SIgA)