Lactoferrin Antikörper (FITC)

Produktnummer ABIN458280

Der FITC-konjugierte Ziege Polyklonal anti-Lactoferrin Antikörper (ABIN458280) detektiert spezifisch Lactoferrin in ELISA, ICC, IHC (fro) und IFA. Dieser Antikörper reagiert spezifisch mit Proben aus Affe.

Kurzübersicht für Lactoferrin Antikörper (FITC) (ABIN458280)

Target: Lactoferrin (LTF) (Lactotransferrin (LTF))

Reaktivität: Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Lactoferrin Antikörper ist konjugiert mit FITC

Applikation: ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Indirect Immunofluorescence Assay (IFA)

Produktdetails anti-Lactoferrin Antikörper (FITC)

Verwendungszweck: Goat anti Monkey lactoferrin, conjugated with FITC

Spezifität: Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Goat antiSerum to Monkey lactoferrin.

Kreuzreaktivität (Details): The antiSerum does not cross-react with any other Monkey plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma or milk proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, however a strong cross-reaction with lactoferrin in Human milk has been observed.

Produktmerkmale: In immunoelectrophoresis against rhesus monkey milk a single precipitin line is obtained. The antiserum does not react with any other protein component of monkey serum or plasma. As reagent for the direct detection of lactoferrin in monkey cells, tissues and body fluids in immunofluorescence, as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA). This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Aufreinigung: Purified IgG fraction from antiserum

Immunogen: Exocrine organs produce various secretions, each with its characteristic function. Proteins found in secretions may be divided into two groups: those specific for the particular secretion, and plasma proteins independent of the type of exocrine cells. Lactoferrin belongs to the first group. It is an iron containing protein with a molecular weight of 75,000 and it is antigenically different from transferrin. Lactoferrin has a slight antimicrobial action. Originally identified in milk, its presence has also been demonstrated in other secretions as saliva, semen and tears. The immunogen has been isolated from monkey milk. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence. The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -20 °C. It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Fluorochrome-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) No preservative added, as it may interfere with the antibody activity.

Konservierungsmittel: Without preservative

Details zu Lactoferrin

Target: Lactoferrin (LTF) (Lactotransferrin (LTF))

Andere Bezeichnung: Lactoferrin

Pathways: Transition Metal Ion Homeostasis